experimentation wild type wt female c57bl 6 mice  (Charles River Laboratories)

Journal: bioRxiv

Article Title: Biofilm-Derived Curli and Z-DNA Shape Anti-DNA Antibody Responses During Salmonella Infections

doi: 10.64898/2026.03.02.708989

Figure Lengend Snippet: (A) Biofilms were grown on glass cover slips and stained with Syto9 (gray), anti-Z-DNA antibody (red), anti-B-DNA antibody (blue), and FSB amyloid stain for curli (green). Images were acquired at 100X using confocal microscopy and merged in ImageJ. White arrows indicate examples of colocalization. (B) Purified curli (green) isolated from in vitro 72-hour STm biofilms was stained for both Z-DNA (red) and B-DNA (blue). (C-D) C57BL/6 mice were i.p. injected with purified curli:DNA (100 µg), or sterile PBS for controls, weekly for 13 weeks and serum diluted 1:400 was analyzed by ELISA for (C) anti-Z-DNA [brominated poly(dGdC)] and (D) anti-B-DNA (calf thymus DNA) antibodies. (E) C57BL/6 mice were i.p. injected with genomic DNA extracted from STm; purified curli:DNA preparations containing an estimated 50μg curli and 45ng DNA treated with DNAse I, Benzonase, or untreated with nuclease post-purification; or PBS for controls, twice per week for 5 weeks. Serum was diluted 1:400 and analyzed by ELISA for anti-Z-DNA antibodies.

Article Snippet: Male and female C57BL/6 (wild type) mice were purchased from Jackson Labs at 4-6 weeks old.

Techniques: Staining, Confocal Microscopy, Purification, Isolation, In Vitro, Injection, Sterility, Enzyme-linked Immunosorbent Assay